Detailed view for TGME49_257720

Basic information

TDR Targets ID: 258789
Toxoplasma gondii, proton ATPase, putative
Source Database / ID:  ToxoDB 

pI: 8.3424 | Length (AA): 2261 | MW (Da): 250493 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 9

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00122   E1-E2 ATPase
PF16209   Phospholipid-translocating ATPase N-terminal
PF16212   Phospholipid-translocating P-type ATPase C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005524   ATP binding  
GO:0004012   phospholipid-translocating ATPase activity  
GO:0000287   magnesium ion binding  
GO:0000166   nucleotide binding  
GO:0015914   phospholipid transport  
GO:0006754   ATP biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
218 279 2hc8 (A) 224 280 33.00 0 0.82 0.324721 0.15
506 706 4hqj (A) 207 379 31.00 0.0014 0.03 -0.146301 2.8
760 882 2mqa (A) 21 139 21.00 0 0.01 0.311701 -0.92
1276 1516 5ksd (A) 366 557 33.00 0.084 0.88 -0.04261 2.09
1321 1510 2b8e (A) 434 612 20.00 0 0.82 0.239334 0.54
1788 1841 5is2 (A) 320 375 37.00 0.16 0.76 0.422683 0.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Tachyzoite. Gregory
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_142437)

Species Accession Gene Product
Babesia bovis BBOV_III007600   phospholipid-translocating P-type ATPase, flippase family protein
Neospora caninum NCLIV_028530   phospholipid-transporting P-type ATPase, putative
Neospora caninum NCLIV_062790   P-type ATPase, putative
Plasmodium berghei PBANKA_0806300   P-type ATPase, putative
Plasmodium falciparum PF3D7_0319000   P-type ATPase, putative
Plasmodium knowlesi PKNH_0822500   P-type ATPase, putative
Plasmodium vivax PVX_095285   potential phospholipid-transporting ATPase, putative
Plasmodium yoelii PY00968   ATPase, P-type, HAD superfamily, subfamily IC, putative
Schistosoma japonicum Sjp_0002680   Probable phospholipid-transporting ATPase IF, putative
Schistosoma mansoni Smp_175790   atpase class VI type 11c
Toxoplasma gondii TGME49_245510   phospholipid-translocating P-type ATPase, flippase subfamily protein
Toxoplasma gondii TGME49_257720   proton ATPase, putative

Essentiality

TGME49_257720 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0806300 Plasmodium berghei Dispensable plasmo
TGME49_257720 this record Toxoplasma gondii Probably essential sidik
TGME49_245510 Toxoplasma gondii Probably essential sidik
TGME49_257720 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_245510 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_257720 (Toxoplasma gondii), proton ATPase, putative
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